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Sample submission guide – bulk repertoire
Samples can be transferred to iRepertoire for library preparation and sequencing at any stage in the immune repertoire sequencing workflow from nucleic acid extraction to amplification to sequencing....
View ArticleShipping samples, receiving products, and submitting data
iRepertoire can help with your immune repertoire sequencing project for any and all steps from nucleic acid extraction, library preparation, sequencing (of a library made with iRepertoire’s Reagent...
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Blood collection and sample considerations
There are various sample collection methods researchers can utilize for immune repertoire studies. This document’s purpose is to define, compare, and contrast several methods of sample collection and...
View ArticleCell-sorting Guide
Introduction to single cell immune sequencing Unlike bulk repertoire analysis, single cell sequencing maintains the cognate pairing between receptor chains in individual immune cells. Our single cell...
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Posters
Posters Coupling DNA Barcoding and Multi-Peptide Screening: Unveiling T Cell Receptor and Antigenic Peptide Relationships in Complex Samples Xiaohong Hou M.D. Ph.D. et al., Presented: SITC 2023 View...
View ArticlePooling samples
Introduction Multiplexing (pooling) several samples into a single sequencing run can significantly reduce costs. To allow for multiplexing, each sample is tagged with a unique barcode during...
View ArticleChoosing your read depth
When planning your repertoire sequencing experiment, you’ll need to consider the appropriate read depth for your samples. Sequencing depth/coverage is impacted by the length of the reads, the...
View ArticleChoice of starting template: RNA vs gDNA vs cDNA
Roughly half the papers published so far for immune repertoire sequencing use genomic DNA samples, while the other half use RNA. Although we have primers that work for both sample types, our preferred...
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Beyond template switching
Investigating the full clonal diversity of an immune repertoire requires a uniquely tailored sequencing approach. Most immune sequencing chemistries on the market rely on some variation of a...
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iRepertoire’s iR-Complete Dual Index Primer Kits for RNA and gDNA
iRepertoire’s iR-Complete Dual Index Kits will replace both the iR-Profile and Bulk Primer kits to simplify reagent ordering and promote scalability of your immune sequencing experiments. Dual index...
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